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Vetriani: Microbiological Experiments at Hydrothermal Vents

1. PI-Project Information

Name: Costantino Vetriani
Institution: Rutgers University
Email address: vetriani@imcs.rutgers.edu
Project Title: 1. COLLABORATIVE RESEARCH: MIP: Physiology and molecular ecology of thermophilic nitrate-reducing microorgansisms at deep-sea hydrothermal vents (PI)
2. COLLABORATIVE RESEARCH: Integrated Studies of Biological Community Structure at Deep-Sea Hydrothermal Vents (Co-PI)
NSF-OCE number: 1. 0456676 (PI)
2. 0327353 (Co-PI)
Upcoming cruise ID and dates: R/V Atlantis Jan. 10th, 07 — Feb. 5th, 2007

2. Planned Sampling Requirements

2.1. Types of Samples:

Low T fluids (diffuse flows)
Sulfides
Microbial mats (Basalt, macrofauna, colonization devices, etc.)

2.2. Type of Sample Locality:

Diffuse flows (e.g., Mk8 RESET 06, Mk11/Tam Town, New Flow)
High T vents for sulfide samples (e.g., Q-, P-, IO-, L-vent, etc.)
Microbial mats/filaments on basalt, macrofauna, etc.
Our sampling plan WILL include other localities not listed above

2.3. Seafloor Collection Devices to be Used:

McLane large volume pump (requires elevator deployment) Ti Majors
Stainless steel mesh microbial colonization devices (SS rounds)
Bioboxes

2.4. Seafloor Experiments to be Deployed:

Stainless steel mesh microbial colonization devices (SS rounds; photo attached; number and location of deployments will vary).

3. Planned Duration of Seafloor Experiments:

Three SS rounds currently on the seafloor. Recovery planned during the Jan 07 Atlantis cruise.
SS rounds CV1 and CV2 currently located at Mk8 RESET 06
SS Round CV4 currently located at Mk 11/Tam Town
Other SS rounds will be deployed.

4. Constraints on Your Deployment Plans:

4.1. Will vehicle operations be allowable in your experiment area? If so, with what restrictions for sampling or imaging (e.g., non-invasive observations or mapping).

Please do not disturb currently deployed SS rounds.

4.2. What is the proximity (meters distant) from your experiment(s) that future sampling can be done.

Far enough to prevent disturbance of deployed devices.

4.3. What is the proximity (meters distant) to which other in-situ instrument deployments can be made.

Same as above

4.4. Will you be using acoustic devices or moorings?

Possibly, acoustic release for elevator used to deploy the McLane pump

4.4. Are you looking for other experiments that can be co-located with your experiments?

TAM deployments

4.5. Does your experiment require site protection? To what level of tolerance?

Same as in 4.1 and 4.2

5. Other Issues?

Stainless Steel Mesh Microbial Colonization Devices.
Dimensions and Weight: Diameter: 12cm (4.7 inches)
Height: 5cm (2 inches)
Weight in air: 435g (14 ounces)
Weight in Seawater: 240g (8.5 ounces)

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